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ice cold d pbs  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ice cold d pbs
    Ice Cold D Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ice+cold+d+pbs/PHOSPHATE+BUFFERED+SALINE+DPBS/bio_rxiv__2024__05__09__593359-69-11-22
    Average 99 stars, based on 1 article reviews
    ice cold d pbs - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Suspension:

    Article Title: Double-negative T cells ameliorate psoriasis by selectively inhibiting IL-17A-producing γδ low T cells
    Article Snippet: .. The cell suspension was then filtered through a 70 μm cell strainer, and the digestion was halted by adding ice-cold D-PBS containing 0.1% BSA (Sigma‒Aldrich, USA) and 5 mM EDTA (Invitrogen). .. To enrich for lymphocytes, a three-layer density gradient using Percoll (GE Healthcare) was employed.

    Article Title: Microglial MHC-I induction with aging and Alzheimer's is conserved in mouse models and humans.
    Article Snippet: .. Single-cell suspension Hippocampi were rinsed in ice-cold D-PBS containing calcium, magnesium, glucose, and pyruvate (#14,287–072, Thermo Fisher Scientific), sliced into four sagittal sections on a chilled, metal block and placed into ice-cold gentleMACS C-tubes (#130–093- 237, Miltenyi Biotec), containing 1950 μl of papainbased Enzyme Mix 1. .. For each reaction, Enzyme Mix 1 was created by combining 50 μl of Enzyme P with 1900 μl of buffer Z, while Enzyme Mix 2 was created by combining 10 μl of Enzyme A with 20 μl of buffer Y per reaction, with all reagents included in the adult brain dissociation kit (#130–107-677, Miltenyi Biotec).

    Single Cell:

    Article Title: Microglial MHC-I induction with aging and Alzheimer's is conserved in mouse models and humans.
    Article Snippet: .. Single-cell suspension Hippocampi were rinsed in ice-cold D-PBS containing calcium, magnesium, glucose, and pyruvate (#14,287–072, Thermo Fisher Scientific), sliced into four sagittal sections on a chilled, metal block and placed into ice-cold gentleMACS C-tubes (#130–093- 237, Miltenyi Biotec), containing 1950 μl of papainbased Enzyme Mix 1. .. For each reaction, Enzyme Mix 1 was created by combining 50 μl of Enzyme P with 1900 μl of buffer Z, while Enzyme Mix 2 was created by combining 10 μl of Enzyme A with 20 μl of buffer Y per reaction, with all reagents included in the adult brain dissociation kit (#130–107-677, Miltenyi Biotec).

    Blocking Assay:

    Article Title: Microglial MHC-I induction with aging and Alzheimer's is conserved in mouse models and humans.
    Article Snippet: .. Single-cell suspension Hippocampi were rinsed in ice-cold D-PBS containing calcium, magnesium, glucose, and pyruvate (#14,287–072, Thermo Fisher Scientific), sliced into four sagittal sections on a chilled, metal block and placed into ice-cold gentleMACS C-tubes (#130–093- 237, Miltenyi Biotec), containing 1950 μl of papainbased Enzyme Mix 1. .. For each reaction, Enzyme Mix 1 was created by combining 50 μl of Enzyme P with 1900 μl of buffer Z, while Enzyme Mix 2 was created by combining 10 μl of Enzyme A with 20 μl of buffer Y per reaction, with all reagents included in the adult brain dissociation kit (#130–107-677, Miltenyi Biotec).

    Cell Culture:

    Article Title: Single cell RNA sequencing reveals immunomodulatory mechanism of stem cell factor and granulocyte colony-stimulating factor treatment in the brains of aged APP/PS1 mice
    Article Snippet: 25021-403-04) Dulbecco’s phosphate-buffered saline (D-PBS; 14190136; ThermoFisher). .. Each brain was removed, immersed in a cell culture dish containing ice-cold D-PBS+ (D-PBS supplemented with calcium, magnesium, glucose and pyruvate; 14287080; ThermoFisher), and minced (∼1-2 mm in any dimension) using an ice-cold sterile scalpel blade. .. Brains were dissociated using the commercially-available Adult Brain Dissociation Kit (130-107-677; Miltenyi Biotec) and the Gentle MACS Octo Dissociator with Heaters (130-096-427; MB) according to manufacturer’s specifications.

    Sterility:

    Article Title: Single cell RNA sequencing reveals immunomodulatory mechanism of stem cell factor and granulocyte colony-stimulating factor treatment in the brains of aged APP/PS1 mice
    Article Snippet: 25021-403-04) Dulbecco’s phosphate-buffered saline (D-PBS; 14190136; ThermoFisher). .. Each brain was removed, immersed in a cell culture dish containing ice-cold D-PBS+ (D-PBS supplemented with calcium, magnesium, glucose and pyruvate; 14287080; ThermoFisher), and minced (∼1-2 mm in any dimension) using an ice-cold sterile scalpel blade. .. Brains were dissociated using the commercially-available Adult Brain Dissociation Kit (130-107-677; Miltenyi Biotec) and the Gentle MACS Octo Dissociator with Heaters (130-096-427; MB) according to manufacturer’s specifications.

    Clinical Proteomics:

    Article Title: Limitations of PLX3397 as a microglial investigational tool: peripheral and off-target effects dictate the response to inflammation.
    Article Snippet: After disappearance of paw and tail reflexes, whole blood was isolated using heart puncture, collected in Microvette 500 K2 ethylenediaminetetraacetic acid (EDTA) tubes (Sarstedt, 20.1339.100) and stored at 4°C until analysis. .. Afterwards, mice were transcardially perfused using 10 ml 0.2% heparin (Sigma, H‐ 3125) in ice-cold D‐PBS (Gibco, 14190‐094) per mouse (4.50 ml/ min), followed by brain, cerebrospinal fluid (CSF) and liver sample isolation. .. Analysis of BBB and blood-CSF barrier integrity Fifteen minutes before sacrifice, mice were injected intravenously (IV) with 250 mg/kg FITC-labelled dextran (4 kDa, Sigma).

    Article Title: Limitations of PLX3397 as a microglial investigational tool: peripheral and off-target effects dictate the response to inflammation
    Article Snippet: After disappearance of paw and tail reflexes, whole blood was isolated using heart puncture, collected in Microvette 500 K2 ethylenediaminetetraacetic acid (EDTA) tubes (Sarstedt, 20.1339.100) and stored at 4°C until analysis. .. Afterwards, mice were transcardially perfused using 10 ml 0.2% heparin (Sigma, H‐3125) in ice-cold D‐PBS (Gibco, 14190‐094) per mouse (4.50 ml/min), followed by brain, cerebrospinal fluid (CSF) and liver sample isolation. .. Fifteen minutes before sacrifice, mice were injected intravenously (IV) with 250 mg/kg FITC-labelled dextran (4 kDa, Sigma).

    Isolation:

    Article Title: Limitations of PLX3397 as a microglial investigational tool: peripheral and off-target effects dictate the response to inflammation.
    Article Snippet: After disappearance of paw and tail reflexes, whole blood was isolated using heart puncture, collected in Microvette 500 K2 ethylenediaminetetraacetic acid (EDTA) tubes (Sarstedt, 20.1339.100) and stored at 4°C until analysis. .. Afterwards, mice were transcardially perfused using 10 ml 0.2% heparin (Sigma, H‐ 3125) in ice-cold D‐PBS (Gibco, 14190‐094) per mouse (4.50 ml/ min), followed by brain, cerebrospinal fluid (CSF) and liver sample isolation. .. Analysis of BBB and blood-CSF barrier integrity Fifteen minutes before sacrifice, mice were injected intravenously (IV) with 250 mg/kg FITC-labelled dextran (4 kDa, Sigma).

    Article Title: Limitations of PLX3397 as a microglial investigational tool: peripheral and off-target effects dictate the response to inflammation
    Article Snippet: After disappearance of paw and tail reflexes, whole blood was isolated using heart puncture, collected in Microvette 500 K2 ethylenediaminetetraacetic acid (EDTA) tubes (Sarstedt, 20.1339.100) and stored at 4°C until analysis. .. Afterwards, mice were transcardially perfused using 10 ml 0.2% heparin (Sigma, H‐3125) in ice-cold D‐PBS (Gibco, 14190‐094) per mouse (4.50 ml/min), followed by brain, cerebrospinal fluid (CSF) and liver sample isolation. .. Fifteen minutes before sacrifice, mice were injected intravenously (IV) with 250 mg/kg FITC-labelled dextran (4 kDa, Sigma).



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